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Chip Reagents
Several reagents have to be added to the chip to prepare it for measurement. The
following image shows which reagents have to be filled in which wells.
Make sure you follow these directions when preparing the sample:
• The priming solution has to be added first. It fills all channels (removes all air from the
micro channels).
• The focusing dye is used to adjust the optic. The optics are focused horizontally and
vertically before each chip is measured.
• If you do not use all six wells, always load the first sample in well 1, the second sample
in well 2 etc. Unused wells have to be filled with cell buffer solution, otherwise they may
run dry during the chip run. Because all channels are connected to the priming well, this
may led to bubbles and to a clogging of the pressure adapter filter.
• The cell buffer is used to focus the cells before they pass the detection point. You have
to fill both buffer wells with cell buffer.
Priming solution
Cell buffer
Sample 1 – 6
Cell buffer
Focusing dye
solution
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