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End Point
The peak find algorithm looks for a leveling off when the value of the slope is less than
the value set for the slope threshold. This is considered to be the end point of the peak.
With RNA assays, individual peak end times can be moved manually by dragging the
diamond-shaped end points shown in the single-well display.
End Time
This setpoint determines the time after the start of a run before which the last peak or
fragment will be located (any peaks appearing after this time are ignored). In RNA
assays, the end time is shown on the single-well display as a long-dashed vertical green
line.
With RNA assays, another End Time setpoint is available that controls the end time for
an individual peak. Additionally, individual peak end times can be moved manually by
dragging the diamond-shaped end points shown in the single view.
F
Filter Width
This setpoint determines the width of the polynomial (in seconds) to be applied to the
data for filtering (noise reduction). The default depends on the assay selected. This
setting should be less than twice the width of the peaks of interest or the peaks will be
distorted. Peaks that are distorted by the filter have positive and negative peaks on both
sides. To see an example of such distortion, increase the filter width to 5.
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