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S
Serial port
The serial ports (COM ports) are used to connect your computer with the Agilent 2100
bioanalyzer. The number of available ports depends on the computer you use.
Signature
Signatures are available in the 2100 expert software only with the security pack installed.
All activities on data such as creating, modifying, and deleting data must be confirmed by
the user with an electronic signature (user name and password). By requesting this
signature it is ensured that only authorized users can create, modify, and delete data.
Slope Threshold
The Slope Threshold setpoint determines the difference in the slope that must occur in
order for a peak to begin. The inverse of this value is used to determine the peak end.
Standard Curve
The standard curve is obtained by plotting the size of the ladder peaks vs. time using a
point-to-point fit. For each sample peak, the center time is interpolated from the Standard
Curve to determine the peak size in base pairs.
Start Point
The peak find algorithm walks the data from time zero looking for a slope greater than the
Slope Threshold. This is considered to be the start point of a peak.
With RNA assays, individual peak start times can be moved manually by dragging the
diamond-shaped start points shown in the single view.
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