Contents ▲ 92 ▼ Index
NOTE
Until now, the computation of the RIN has only been validated for eukaryote total RNA
Nano samples. The 2100 expert software also calculates the RIN for prokaryote total
RNA samples and for the RNA 6000 Pico assay. Be aware that for these samples, the
RIN has not been validated in extensive downstream experiments.
Although the lower quantitative limit of the RNA 6000 Nano assay is specified as 25
ng/µl it is recommended to use at least 50 ng/µl for a meaningful RNA integrity number.
When using lower concentrations, higher sample to sample variances of the RIN may be
observed.
Examples for RNA Integrity Numbers
A database of about 1300 mammalian total RNA samples was created using the RNA
6000 Nano assay. The samples came from different species (mainly human, rat and
mouse), tissues, preparation methods, concentrations and degradation states. All
samples were classified according to their degradation state. Numbers from ’1’ to ’10’
were used as labels. ’10’ stands for a perfect RNA sample without any degradation
products, whereas ’1’ marks a completely degraded sample. The labels in-between are
used to indicate progressing degradation states of the RNA sample. The following figure
shows typical representatives for each of the 10 RNA integrity classes.
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