Exercise 1 – Develop an acquisition method
Task 3. Find optimum fragmentor voltage for maximum response
Agilent 6400 Series Triple Quad LC/MS Familiarization Guide 15
b In the Acquisition tab, enter the
Compound Name and Mass
(precursor ion mass) for
sulfadimethoxine (311 m/z).
c Right-click anywhere in the Scan
segments section, and click Add Row.
d Type the Compound Name and the
Mass for sulfachloropyridazine (285
m/z).
e Repeat steps c and d for
sulfamethazine (279 m/z) and
sulfamethizole (271 m/z).
f Save the method as iiiMS2SIM140.m,
where iii are your initials.
g Change the fragmentor voltage to 60,
and save the method as
iiiMS2SIM060, where iii are your
initials.
h Repeat step g for voltages 80, 100, 180
and 220, saving the methods as
iiiMS2SIM080, iiiMS2SIM100,
iiiMS2SIM180 and iiiMS2SIM220,
where iii are your initials.
• With the MS2SIM Scan Type set, a
different set of columns appears in
the Acquisition window.
• The Instrument Control and Data
Acquisition program creates a SIM
experiment for each compound
mass, starting with a default
fragmentor voltage of 140. See the
example below.
Steps Detailed Instructions Comments
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