Exercise 1 – Develop an acquisition method
Task 3. Find optimum fragmentor voltage for maximum response
16 Agilent 6400 Series Triple Quad LC/MS Familiarization Guide
2 Set up and run the worklist
(optional).
• Set up six samples with Sample
Name SulfaDrugMix to inject 1ul
from vials 1-6 or the ones you
choose.
• Specify the data files as
iiiSulfaSIMxxx.d, where iii are
your initials and xxx is the
voltage.
a Click the Worklist icon if necessary to
make sure the worklist is visible.
b Click Worklist > New to start a new
worklist. You do not need to save the
last worklist.
c To set up the run, right-click the upper
left corner of the worklist, and click
Worklist Run Parameters.
d Type the paths for the method and
data files.
e Type the information for the 60 voltage
run.
f Click Worklist > Add Sample.
Another sample is added to the
Worklist. Add five samples to the
worklist for voltages 80-220.
g Mark the checkbox to the left of the
Sample Name for each of the six
samples.
• This step is optional because you
can use data files shipped with the
system to perform many of the
tasks in this exercise.
h Start the worklist.
• Click Worklist > Run.
• Click the icon in the main
toolbar.
• Click the icon in the worklist
toolbar.
• Note that the program only runs
those samples that are marked with
a checkmark.
• You can also run the worklist in
locked mode by clicking the lock
button in the main toolbar (the icon
looks like this icon when it is
locked).
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