Exercise 1 – Develop an acquisition method
Task 4. Determine product ion masses
Agilent 6400 Series Triple Quad LC/MS Familiarization Guide 25
3 Set up a qualitative method to
integrate and extract product ion
spectra.
• Use the data files
SulfamixPI_xx.d, where xx is
the collision energy, or your own
data files, iiiSulfamixPI_xx.d.
• Open Method Explorer and
Method Editor.
• Use TICs set up for MS/MS,
product ion and each of the
precursor ions 271, 279, 285,
311.
• Make sure the MS/MS
integrator has been selected and
the maximum number of peaks
has been limited to the largest
100 peaks.
• Add the ability to integrate and
extract peak spectra to the file
actions run upon data opening.
• Save the changes to the current
method.
a Click the Open Data File icon in the
toolbar.
b Select SulfamixPI_15.d.
c Clear the Run File Open Actions from
Specified Method check box, and
click Open.
d Make sure the Method Explorer and
the Method Editor windows are
displayed; otherwise, click the Method
Explorer and then Method Editor
icons.
e In the Chromatogram section in the
Method Explorer window, select
Define Chromatograms.
f Delete any existing chromatograms in
the Defined Chromatograms list.
g Select TIC from the Type list in the
Define chromatograms section.
h For MS level, select MS/MS.
i Mark the Do cycle sum check box.
j For Scans, select Product ion.
k For Precursor ion m/z, type
271.
l Click the Add button.
m Repeat steps j and k for each ion.
• The Qualitative Analysis program
should already be open and contain
iiiexercise 1.m as the method.
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