Exercise 3 – Create a Triggered Dynamic MRM acquisition method
Task 2. Add/Modify compounds in an existing database
Agilent 6400 Series Triple Quad LC/MS Familiarization Guide 47
• To determine product ions in the
Qualitative Analysis program:
a Open the SulfamixPI_15.d from “Task
4. Determine product ion masses” on
page 24.
b Click Find > Find Compounds by
Targeted MS/MS.
c Close the Compound List window.
d Select a compound in the Data
Navigator window. For this example,
click Cpd 4.
e Click the Autoscale Y-axis icon in the
MS Spectrum Results toolbar.
f Right-click and drag to zoom in on the
MS spectrum.
• If possible, rearrange the windows
on the screen so you can see the
Optimizer program and the
Qualitative Analysis program at the
same time.
• See Table 5 on page 54 for values to
use in the method.
7 Set other parameters in the
Compound Setup tab and start the
optimization.
• You cannot perform a
multi-compound run.
• You have to mark each row in
the table to use.
a Mark the check box in the left column
at the top of the table. The check box
for every row in the table is marked.
b Clear the Perform multi-compound
run check box in the right column.
c Click the Start Optimization button in
the Optimizer toolbar.
• You cannot perform a
multi-compound run with the
number of transitions that were
added. If you mark this check box,
then the Expected peak width
(base) is automatically set to
almost 80 seconds wide. If you
clear this check box, then the
Expected peak width is calculated
to be around 9 seconds which is
more appropriate.
• See Table 5 on page 54 for values to
use in the method.
Steps Detailed Instructions Comments
This product ion scan has a
precursor mass of 311. You
examine the MS spectrum to
determine the product ions
to add to the Product ion
section of the Compound
Setup table.
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