Exercise 3 – Create a Triggered Dynamic MRM acquisition method
Task 2. Add/Modify compounds in an existing database
48 Agilent 6400 Series Triple Quad LC/MS Familiarization Guide
8 Examine the Optimizer Report. a Examine the Collision Energy for each
Product Ion.
b Print or save the report.
9 Save the compounds. • Click the File > Save Compounds
command.
10 Import compounds from a
database.
• Click the Import/Export > Import from
Database command. The Database
Browser program opens.
• You can also import compounds
that were distributed as part of a
database.
Steps Detailed Instructions Comments
As a general rule, as the Product Ions get smaller,
the optimal Collision Energy gets larger. However,
when you also examine the abundance, you can
see that if the Collision Energy is set to 48 for the
smallest product ion, the smallest product ion can
become the dominant peak. The collision energies
are further adjusted later in this task.
This report was generated with a different set of
transitions.
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