Operation Guide
Preparing The Solvent And Waste Bottles
13
Preparing The Solvent And Waste Bottles
Solvent bottles hold solvent for rinsing the syringe between injections. The
injector discards the solvent washes and sample washes into waste bottles. The
number of samples that can be analyzed may be limited by the wash or waste
bottle capacity shown in Figure 7 and Figure 8.
Rinse and fill each solvent bottle with 4 to 4.5 mL of fresh solvent. The liquid
level will be near the shoulder of the bottle. Good laboratory practice dictates
using no more than 2.0 mL of the 4.5 mL solvent for syringe washes. The needle
tip draws solvent at a height of 18.5 mm from the bottom of the vial. See Figure 7.
Figure 7 Position of syringe tip when withdrawing solvent
Empty and rinse each waste bottle after each multiple vial run. The syringe can
discard about 4 mL of waste into the waste bottle. See Figure 8.
Figure 8 Position of syringe tip when discarding waste
500 washes of a 5 µL syringe
18.5 mm
4.5 mL fill volume
2.5 mL solvent remains
2.0 mL usable solvent volume
250 washes of a 10 µL syringe
100 washes of a 25 µL syringe
50 washes of a 50 µL syringe
25 washes of a 100 µL syringe
MINIMUM
SOLVENT
LEVEL
Capacity of one solvent bottle
4 mL maximum waste
500 washes of a 10 µL syringe
1000 washes of a 5 µL syringe
200 washes of a 25 µL syringe
100 washes of a 50 µL syringe
50 washes of a 100 µL syringe
Capacity of one waste bottle
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