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Agilent Technologies 7100 User Manual

Agilent Technologies 7100
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Agilent Technologies
Agilent 7100
Capillary Electrophoresis
System
User Manual

Table of Contents

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Agilent Technologies 7100 Specifications

General IconGeneral
BrandAgilent Technologies
Model7100
CategoryLaboratory Equipment
LanguageEnglish

Summary

1 Introduction

Introduction to the Agilent 7100 Capillary Electrophoresis System

Overview of the Agilent 7100 CE System and how to get started.

Getting Help

Information on how to access and use the online help system for assistance.

Where to Find Information

Guidance on locating specific information and tasks within the manual and online help.

What Learning Products are Available?

Details on available learning resources and documentation for the CE system.

2 Site Requirements and Specifications

Site Requirements

Information on environmental and physical requirements for instrument installation.

Power Consideration

Details on power supply capabilities, voltage, and safe power connection practices.

Power Cords

Information regarding the types of power cords supplied and their proper use.

Bench Space

Requirements for instrument placement, including space for air circulation and connections.

Environment

Specifications for ambient temperature and humidity for optimal instrument operation.

Physical Specifications

Technical specifications detailing the instrument's physical characteristics and dimensions.

Performance Specifications

Details on the instrument's performance capabilities and features.

3 Installing the Instrument

Unpacking the Agilent CE Instrument

Step-by-step instructions for safely unpacking the Agilent CE instrument.

Damaged Packaging

Procedure to follow if the instrument packaging shows signs of external damage.

4 LAN Configuration

What You Have to Do First

Initial steps and options for connecting the CE instrument to a LAN.

TCP/IP Parameter Configuration

Details on configuring the necessary TCP/IP network parameters for LAN connection.

Configuration Switch

Information on using the physical configuration switch for LAN setup.

Initialization Mode Selection

Options for selecting the instrument's initialization mode for network connection.

Link Configuration Selection

Manual settings for LAN link speed and duplex mode.

Storing the Settings Permanently with Bootp

Procedure to save network configuration parameters using the Bootp service.

Manual Configuration

Steps for manually configuring network settings via Telnet or other methods.

Agilent Bootp Service

Explanation of the Agilent Bootp Service for IP address administration.

Location of the Media Access Control (MAC) Address

How to find the instrument's unique MAC address for network identification.

5 Using the Agilent 7100 Capillary Electrophoresis System

System Definition

Overview of the system components: CE instrument, ChemStation, and Lab Advisor software.

Controlling the Agilent CE Instrument

Guidance on the procedures for starting up and operating the Agilent CE instrument.

Starting Up the Agilent CE Instrument

Step-by-step instructions for powering on and initializing the CE instrument.

Starting Your Computer and the Agilent ChemStation Software

Procedure for starting the computer and the ChemStation software.

Starting the Agilent ChemStation Software the First Time

Initial setup steps for ChemStation software, including configuration.

Working with the CE Diagram

Explanation of the CE diagram interface for monitoring instrument components.

Common Software Toolbar

Description of the functions available on the ChemStation software toolbar.

Graphical User Interface Explanation

Detailed explanation of the CE diagram components and their icons.

Menus in the CE and DAD Diagram

Accessing instrument control and method parameters via context menus.

Direct Control of Instrument Components

Performing immediate actions on instrument components through the CE diagram.

Set Error Method

Storing parameters to ensure controlled shutdown during errors.

Identify Device

Command to locate the instrument using its status indicator LED.

System Vialtable

Using the vialtable for managing vials and checking method/sequence consistency.

How to Prepare your Agilent CE Instrument for Analysis

Tasks required to prepare the CE instrument for running an analysis.

Capillary Electrophoresis, CE

Introduction to running applications in CE mode and its suitability.

Agilent CE Method Parameters

Description of method parameters for data acquisition and analysis.

Vials

Programming methods to use specific vials for inlet or outlet home positions.

Cassette Temperature

Setting and controlling the temperature of the capillary cassette.

High Voltage System

Controlling parameters of the power supply, including voltage, current, and power.

Voltage, Current and Power

Setting voltage, current, and power values as limits for the system.

Lower Alarm Limit for Current

Setting a lower limit for current to warn of system instability.

Posttime and Stoptime

Defining separation time and post-analysis equilibration time.

Replenishment

Managing buffer composition by automatically refilling buffer vials.

Preconditioning

Steps to condition capillaries for stable analysis conditions.

Wash Inlet Electrode (New Command to Avoid Carry-over in Critical Applications)

Using a command to empty space between capillary and electrode to prevent carry-over.

Injection

Choices for CE injection techniques: hydrodynamic, electrokinetic, or program.

Hydrodynamic Injection

Procedure for sample injection using pressure or vacuum.

Electrokinetic Injection

Procedure for sample injection using applied voltage, current, or power.

Difference Between Hydrodynamic and Electrokinetic Injection

Comparison of hydrodynamic and electrokinetic injection methods.

Using an Injection Program

Advanced injection tasks using the injection table and timetable.

Timetable

Programming parameters at specific times during the run.

Instrument Curves

Monitoring and storing CE-specific raw data like leak current and pressure.

Detector Parameters

Setting parameters for the detector, including signals, wavelengths, and peak width.

Signals, Wavelengths and Bandwidths

Defining detector signals, their wavelengths, and bandwidths.

Peak Width

Setting the optimum response time for the detector based on peak widths.

Spectra

Defining points for taking and saving spectra during analysis.

Detector Stoptime and Posttime

Defining special stoptime and posttime for the detector.

Analog Output

Setting up the zero offset and attenuation of the analog output signal.

Indirect UV

Setting a margin for peaks with negative absorbance.

Autobalance

Zeroing the baseline of the detector before or after an analysis.

Lamps On Required for Acquisition

Option to save lamp life by deselecting lamp use if a different detector is configured.

Detector Timetable

Programming detector parameters like wavelength and peak width over time.

6 Troubleshooting and Diagnostics

CE Problem Solving

General guidance for solving common instrument failures and issues.

Power On Fail

Troubleshooting steps for when the instrument fails to power on.

Leaks

Information about the leak sensor and actions to take if a leak is detected.

Blocked Replenishment Needle

Troubleshooting steps for a blocked replenishment needle due to buffer precipitation.

Leakage Current

Understanding and troubleshooting leakage current issues in the instrument.

Broken Capillary

Identifying and resolving issues related to a broken capillary.

Contaminated Insulation Plate

Troubleshooting leakage current caused by contamination of the insulation plate.

Empty Injection Vial

Checking if an injection vial is filled when encountering issues.

Dirt in the Vials

Instructions for cleaning vials to prevent contamination.

Air Bubble in Injection Vial or Run Buffer Vials

Addressing air bubbles in sample or buffer vials that can cause injection issues.

Air Bubble in Capillary

Troubleshooting air bubbles within the capillary that affect electropherograms.

Problems with the Replenishment System and Buffers Containing SDS

Resolving issues related to foam formation in SDS buffers within the replenishment system.

Agilent Lab Advisor Software

Overview of the Agilent Lab Advisor software for diagnostics and maintenance.

Configuration

Steps for configuring the Lab Advisor software to communicate with the CE instrument.

7 Hardware Information

Identifying Instrument Components

Identification of the main physical components of the Agilent CE instrument.

7100 Capillary Electrophoresis Instrument Electronics

Detailed information about the instrument's electronic components and connectors.

Fuses

Information on fuse types, locations, and replacement procedures.

Firmware

Description of the instrument's firmware structure (resident and main systems).

Firmware Updates

Procedure for updating the instrument's firmware using software tools.

Diode Array Detector

Information on controlling the Diode Array Detector and its parameters.

DAD Control

Using the DAD control dialog in ChemStation to manage the UV lamp and analog output.

Changing the Fuses for the Power Supplies

Step-by-step guide for replacing fuses in the instrument's power supplies.

Installing Drainage Tubing

Procedure for installing drainage tubing to collect instrument condensation.

External Water Bath for Tray Cooling (Optional)

Information on connecting and using an external water bath for sample tray cooling.

Analog to Digital Converter

Explanation of the built-in AD converter for analog signal processing.

8 Maintenance

Standard Operating Procedures for Maintenance of Your Agilent CE Instrument

General SOPs for regular maintenance tasks on the Agilent CE instrument.

Overview of Maintenance

Summary of maintenance procedures, typical frequencies, and important notes.

Cleaning the electrodes, pre-punchers and insulation plate

Procedure for cleaning electrodes, pre-punchers, and the insulation plate regularly.

Prepare the Agilent CE Instrument

Steps to prepare the instrument before performing maintenance tasks.

Remove the Covers

Instructions for safely removing the instrument covers to access internal components.

Accessing the Electrodes

Procedure for accessing the instrument's electrodes for cleaning or inspection.

Accessing the Liquid Handling Module

Steps to access the liquid handling module for maintenance or inspection.

Accessing and Removing the Pre-punchers

Procedure for accessing and removing the pre-puncher components.

Cleaning the Electrodes

Detailed steps for cleaning the instrument's electrodes.

Cleaning the Pre-punchers

Detailed steps for cleaning the instrument's pre-punchers.

Cleaning the Insulation Plate

Detailed steps for cleaning the instrument's insulation plate.

Reinstalling the Pre-punchers

Procedure for correctly reinstalling the pre-punchers after cleaning.

Reinstalling the Electrodes and the Insulation Plate

Steps for reinstalling the electrodes and insulation plate after maintenance.

Reinstall the Capillary Cassette

Instructions for reinstalling the capillary cassette into the instrument.

Reinstall the Front Cover

Procedure for reinstalling the instrument's front cover.

Maintenance of the Replenishment System

Procedures for maintaining the buffer replenishment system.

Changing the Buffer Composition in the Replenishment System

Steps for changing the buffer solution in the replenishment system.

The Replenishment System Will Not Be Used for Some Time

Procedure for flushing and storing the replenishment system when not in use.

Further Action

Troubleshooting steps if the instrument fails to build pressure or vacuum.

Operations for Replenishment Maintenance

Starting replenishment maintenance operations via software.

Flush Tubes

Operation to flush cleaning solutions through the replenishment tubing.

Clean Tubes

Operation to replace liquid in tubing and fill/empty vials for cleaning.

Clean Level Sensor

Function to dissolve buffer crystals and clean the level sensing needle.

Changing the Air Inlet Filter

Procedure for replacing the air inlet filter for the instrument's air pump.

Accessing the Air Filter

Location and replacement of the air inlet filter.

Changing the Lamp

Procedure for replacing the deuterium lamp in the instrument.

Removing the Existing Lamp

Steps for safely removing the old deuterium lamp.

Inserting a New Lamp

Procedure for installing a new deuterium lamp.

Cleaning the Instrument

Guidelines for cleaning the exterior of the instrument case.

9 Parts and Materials for Maintenance

Overview of Maintenance Parts

List of parts and materials required for instrument maintenance.

10 Appendix

General Safety Information

General safety precautions to be observed during all phases of instrument use.

Setting Up a Test Sample Run

Procedure for setting up and running a test sample for instrument verification.

Chemical and Biological Safety

Safety procedures for handling toxic, hazardous, or biological samples and solvents.

The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC)

Information regarding the WEEE Directive for proper disposal of electronic waste.

Radio Interference

Guidelines on using appropriate cables to ensure EMC compliance.

Sound Emission

Manufacturer's declaration regarding the product's sound pressure emission levels.

Legal Notice

Information regarding the limited right to use the instrument and patented technologies.

Agilent Technologies on Internet

Information on accessing Agilent's website for product and service updates.

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