252 Agilent 7100 Capillary Electrophoresis System User Manual
10 Appendix
Setting Up a Test Sample Run
Setting Up a Test Sample Run
This instrument check demonstrates that the instrument is correctly installed
and connected. Please use a fused silica capillary with 40 cm effective length
and 50 µm inner diameter from the HPCE start-up capillary kit (part number
G1600-68700) with the green alignment interface (part number G7100-60210).
The instrument should be in CE mode.
Load chemicals from the Installation Qualification Chemical kit (part number
5063-6514) according to the table below. The Installation Qualification
Chemical kit contains:
• Buffer (20 mM borate, pH 9.3, 100 mL)
• Test sample (4-hydroxyacetophenone 1 mM, 3 mL)
• Conditioning solution (1.0 N sodium hydroxide, 100 mL)
Choose the menu item Method > New method to prepare a method with the
parameters given below. Save the edited method under a new name. Choose
the menu item RunControl > Sample Info… and enter the operator name,
directory and file name. Enter Vial location 7, enter sample name
“1 mM pHAP” and click Run method to start the analysis.
A typical electropherogram will show a significant peak at a migration time of
around 3 minutes.
Table 34 Solution for Checkout Run
Location Solution Volume
Vial 1 1.0 N sodium hydroxide 500 µL
Vial 2 Water 200 µL
Vial 4 Buffer 500 µL
Vial 5 Buffer 500 µL
Vial 6 Buffer 500 µL
Vial 7 Test sample 500 µL
Vial 8 Water/waste 200 µL
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