Agilent 7100 Capillary Electrophoresis System User Manual 111
Using the Agilent 7100 Capillary Electrophoresis System 5
Capillary Electrophoresis, CE
Preparing the Replenishment System if Needed
During multiple analyses, the composition of the buffer can change. One way
to refresh the buffer is replenishment.
The replenishment system automatically empties and refills the buffer vials in
the tray with new buffer from the electrolyte bottle. Typically, replenishment
is done before every or after every 3 to 4 runs, depending on the stability and
volume of the running buffer.
If you do NOT want to use the replenishment system, you still have to put
empty bottles (electrolyte and waste) into the replenishment system at the
bottom of the instrument. The bottles are also used as a pressure and vacuum
reservoir. Missing bottles lead to pressure and/or vacuum error messages.
If you want to use the replenishment system, you have to fill the electrolyte
bottle with buffer. Use about 100 mL to make sure the frit is submerged in
buffer. Do not fill more than 400 mL buffer into the bottles to ensure the
system can work properly. When using buffers containing detergent (for
example SDS), the waste bottle should contain some liquid (for example a few
mL of isopropanol) to destroy the detergent bubbles. Detailed information on
buffers is given in the primer High Performance Capillary Electrophoresis
(5990-3777EN), delivered together with the instrument or numerous
application notes available on our web page.
Make sure the waste bottle is empty so it can hold the same amount of liquid
as in the electrolyte bottle.
System under pressure
Pressure is applied to the bottles.
• Only use bottles with part number 9300-1748 or 5042-6478 with this instrument.
These bottles have a protective coating.
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