Installation
24 Cary Eclipse Fluorescence Spectrophotometer User’s Guide
Cuvette pitch adjustment
1 Turning the pitch adjustment screw (5) in a clockwise direction
will raise the front of the cell holder.
2 Turning the pitch adjustment screw (5) in an anti-clockwise
direction will lower the front of the cell holder.
NOTE
The pitch adjustment screw requires a 2.5 mm hex key.
Aligning
To align the single cell holder (containing a microcell):
1 Open the Cary Eclipse folder on the desktop and then double-
click Align to start the Align application.
2 Click the Cary tab.
3 Place a fluorescent sample (for example, anthracene) in a
microcell and adjust the excitation and emission wavelengths to
get a signal.
4 Click Apply. (The wavelengths should change and the green
power indicator on the instrument should continue flashing to
indicate that the instrument is active.)
5 Place a small piece of white paper in the light path to the right of
the cell (use a wavelength of 540 nm). If the beam appears as
though it will strike the cell aperture, move the paper to the left
of the cell and check that the beam is passing through the cell. (If
the beam does not appear as though it will pass through the cell,
adjust the height of the cell as described on Page 23).
6 Using the 2.5 millimeter hexagonal ball driver, adjust the pitch
adjustment screw (‘5’ in Figure 2) and note the intensity of the
light striking the paper. Continue to adjust the adjustment screw
until the beam hitting the paper appears the most intense.
NOTE
You may need to dim the room lights to see the light beam.
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