Maintenance and Spare Parts
Cary Eclipse Fluorescence Spectrophotometer User’s Guide 35
Aligning
For optimum performance of the instrument, the lamp module must
be aligned.
To align the lamp module:
NOTE
The instrument power must be on for the alignment procedure.
1 Open the Cary Eclipse folder on the desktop and then double-
click Align to start the ‘Align’ application.
2 Remove the lamp access panel (see Figure 1) to access the lamp
alignment screws.
3 In the Align application, click the Cary tab and set up these
instrument parameters:
Ex. Wavelength (nm): 540
Ex. Slit (nm): 1.5
Ave. time (s): 0.1
PMT voltage: Medium
NOTE
Emission parameters are not important for this procedure.
4 Click the Graph tab.
5 View the reference signal by clicking the Reference Signal button
on the toolbar.
6 Click the Vertical Rescale button to find the signal range.
7 Click the Scale button and enter an upper limit that is 20%
higher than the current signal and a lower limit that is 20% lower
than the current signal. This will make the adjustment easier
when observing the graph.
8 Adjust the bottom left screw (vertical adjustment, labeled ‘1’ in
Figure 5) for maximum signal with the supplied 5.5 millimeter
nut driver (you may need to adjust the y-axis scaling on the
screen).
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