1200 Series DAD and MWD User Manual 139
Maintenance 8
Holmium Oxide Test
The holmium oxide test uses three characteristic absorbance maxima of the
built-in holmium oxide filter to verify wavelength accuracy (see also
“Wavelength Verification and Recalibration” on page 138). When the test is
started, the 1-nm slit is moved into the light path automatically. To eliminate
effects due to absorbing solvents, the test should be done with water in the
flow cell.
Holmium Oxide Test Evaluation
Limits:
The test is evaluated by the instrument, and the measured maxima are
displayed automatically. The test fails if one or more of the maxima lies
outside of the limits (see
Figure 44 on page 140).
Test Failed
Probable Causes
• Absorbing solvent or air bubble in flow cell.
• Incorrect calibration
• Dirty or contaminated flow cell.
• Dirty or contaminated optical components (achromat, windows).
• Old or non-Agilent lamp.
NOTE
See also “Declaration of Conformity for HOX2 Filter” on page 176.
361.0 nm 360.0 - 362.0 nm (± 1nm)
453.7 nm 452.7 - 454.7 nm (± 1nm)
536.7 nm 535.7 - 537.7 nm (± 1nm)
NOTE
The evaluation report is only on the Agilent ChemStation available.
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