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Split Mode Variables, Practices and Rationales
Parameter Selection/Setting Rationale
Inlet temperature Try 250°C or BP of last eluting
c
ompound
Ensures flash vaporization
M
inimizes inlet discrimination
Inlet liner Large volume, deactivated Minimizes flashback
Minimizes degradation
Inlet packing Silanized glass wool
Glass beads or frit
None
Retains non-volatiles
Minimizes inlet discrimination
Less active than wool
Least active
Injection volume 0.5-3 µL liquid
0.10-10 mL gas
Split easily adjusted
Split adjusted accordingly
Injection technique Fast autoinjection
Hot-needle fast manual injection
Less needle discrimination
Reproducible discrimination
Split ratio 50:1 to 500:1 Depends on sample and injection
volume, and column ID
Initial column temperatures Not critical Narrow initial peaks
Septum purge 2-3 mL/min Minimizes ghosting
Split liner
, 5183-4647
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