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10. PIPETTING FOR ACCURATE DISPENSING
□ When performing aspiration, if the tip is immersed
too deeply into the sample liquid, an amount larger
than the selected dispensing volume may be
delivered, as excess liquid attaches the outside of the
tip. Ideally, for aspiration, the tip should be dipped into
the liquid to a depth of 2 to 3 mm.
The pipette is designed to correctly perform
aspiration when it is in the vertical position.
Therefore, hold the pipette as vertically as possible
when aspirating.
□ Be sure to increase the number of pre-rinses when aspirating volatile liquids.
Volatile substances in the tip can lead to lowering in the amount dispensed.
□ When replacing the tip, pre-rinse the tip with the
necessary dispensing volume setting. The reverse
operation is recommended for a sample liquid that
tends to linger in the tip.
※ For accurately dispensing various kinds of liquids, please refer to “Pipette Operation
Guide – for accurate dispensing with pipettes” on the A&D website.
(http://www.aandd.jp/products/test_measuring/pipette/mpa.html)
2 to 3mm
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