Applied Biosystems 7900HT Getting Started Guide

DRAFT
June 23, 2003 9:39 am, MFC_QSG_text.fm
Preparing and Running the Micro Fluidic Cards (PCR)
Applied Biosystems Micro Fluidic Card Getting Started Guide 25
5. In the Instrument tab, make sure that the default thermal
cycling conditions for Micro Fluidic Cards are set:
6. Save the document or continue without saving, as explained
in the following table:
7. Enter the sample names.
Sample names serve as keys to group samples for RQ
analysis.
8. Continue with “Performing the Micro Fluidic Card Run” on
page 26.
To create a Micro Fluidic Card template or document for relative
quantification using the SDS setup file: (continued)
AmpErase
UNG
Activation
AmpliTaq
Gold DNA
Polymerase
Activation
Each of 40 Cycles
Melt Anneal/Extend
50 ° C
2 min
100% ramp
94.5 ° C
10 min
100% ramp
97 ° C
30 sec
50% ramp
59.7 ° C
1 min
100% ramp
If you are running... Then...
a single Micro Fluidic Card go to step 7.
Alternatively, you can save the
document for later use.
the first Micro Fluidic Card in
a series with identical assay
configurations
Note: These instructions are
for running Micro Fluidic
Cards manually. For
instructions on batch
automation, refer to Chapter 6
of the user guide for the
7900HT system.
1. Save the Micro Fluidic
Card document as an SDS
template document (*.sdt).
2. Create a new Micro Fluidic
Card document (*.sds)
from the template.
3. Go to step 7.

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English