Applied Biosystems GeneAmp PCR System 9700 User Guide

DRAFT
October 24, 2007 9:40 am, 44343363 D 03 Overview.fm
Loading Samples
GeneAmp PCR System 9700 Dual 96 Sample Block Module User Guide 1-9
Loading Samples
The following procedures describe how to load samples for:
• Tubes with attached caps
• 96-Well reaction plate
• 96-Well Tray Retainer Assembly
• Sample volume range: 5 to 100 µL in “D96” mode
IMPORTANT! Do not use mineral oil or glycerine in the sample block or as a vapor
barrier over the PCR reaction mixture in the tubes. The MicroAmp Reaction Tubes
fit tightly in the wells and a heated cover exerts even pressure on all tubes, reducing
condensation on the tubes when you are using a tray or tray/retainer set.
Loading Tubes
with
Attached Caps
To load tubes with attached caps:
1. Set the 96-well tray on a 96-well support base.
2. Place the reaction tubes in the tray.
3. Pipette the samples into the reaction tubes.
4. Cap the tubes.
Loading the
96-Well
Reaction Plate
To load the 96-well reaction plate:
1. Place the reaction plate on the 96-well support base.
2. Pipette the samples into the sample wells.
3. Apply the adhesive cover with a compression pad or cap the tubes using either
the MicroAmp 96-Well Full Plate Cover or the MicroAmp 8-Strip Caps.
Loading the
96-Well Tray
Retainer
Assembly
To load the 96-Well Tray Retainer Assembly:
1. Place the tray on the 96-well support base.
2. Load tubes onto the tray, either using single tubes or using the 8-well tubes.
3. Place retainer over the tubes.
4. Pipette the samples into the tubes.
5. Cap the tubes using either the MicroAmp 96-Well Full Plate Cover or the
MicroAmp 8-Strip Caps.

Applied Biosystems GeneAmp PCR System 9700 Specifications

General

Brand	Applied Biosystems
Model	GeneAmp PCR System 9700
Category	Laboratory Equipment
Language	English