Avantik Cryostat QS11 / QS11UV
Avantik
36 Commerce St.
Springfield NJ 07081 / USA
387 779 - English
50
4 WORKING WITH THE
CRYOSTAT
4-1 PREPARING THE COOLING
CHAMBER
Before sectioning, the microtome chamber
should be at a stable temperature around the
desired cutting temperature. The temperature
of the knife is determined by the cooling of the
microtome chamber.
All tools which are necessary to take off
sections or to manipulate the specimen must
also be cooled, as the section will stick to
them. For this reason, the required working
accessories should always be stored on the
shelves or brush tray in the microtome
chamber.
The heated sliding window should be closed
during breaks and while preparing work to
avoid warm air getting into the chamber.
Consequently, more favourable work
conditions with a lower frost built-up on the
microtome and knife are achieved.
4-2
FREEZING-ON THE SPECIME
N
The specimen is frozen-on to the specimen
stage with clinging grooves with a freezing
compound.
To freeze-on specimens, use the fast freezing
device or the Peltier element on the left side of
the chamber.
When using the fast freezing device put some
freezing compound onto the specimen stage,
which should be outside the chamber at a low
positive temperature. Then put the specimen
onto the stage and surround it with some
freezing compound.
Then insert the chuck into one of the fast
freezing stations.
When the Peltier element is used for the
freezing-on, first activate it. For this, briefly
press the down button twice. On the right
temperature indication a "
P" is shown in front
of
the current chamber temperature, which
means that the Pelter element is turned on.
If the instrument is equipped with a heat
extractor (additional equipment) the specimen
can be frozen faster and more evenly. Put the
heat extractor onto the specimen from above.
This way, the specimen is frozen at the same
time from above and below, which reduces the
formation of artefacts considerably.
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