Azure 200 - Fluorescent Labels Imaging Mode; Sample Preparation and Setup; Channel Selection and Imaging

Azure Imaging System User Manual Page 14
3.4 Fluorescent Labels Imaging Mode
Use this for samples with uorescent signals in up to four channels (displayed in red, green, blue, and
grayscale pseudocolor) for multiplex imaging.
1. Remove the black tray from its position on the inside of the door, and place the uorescent blot or gel
in the center of the tray. Then place the tray and sample on the bottom or top shelf inside the imager.
2. Close the Azure Imaging System door.
3. Select Fluorescent Labels from the home screen.
4. Select desired uorescent labels by clicking on any one of four color channels: red, green, blue, and
gray, and selecting the desired dye to assign it to that pseudo-color channel.
Note: List of dyes available is customizable in the Settings tab.
5. Use Auto Image if you want the system to determine the optimal default exposure for each selected
channel using the RapidCapture exposure mode.
6. Select Manually Image to control all imaging settings.
7. Select the Pixel Binning setting based on the desired sensitivity and imaging resolution. See Section
3.2 for more details.
8. Exposure type can be selected for each channel. Select between the different auto exposure modes
or choose Manual Exposure Type to enter the desired exposure time. See Section 3.2 for more details
on the different Auto Exposure options.
9. The info button offers recommendations on imaging and directions on whether a tray is required for
the imaging type chosen.
10. In Manually Image menu, tap to enable Live Mode. Click again to close. Live Mode illuminates
the imaging area to allow you to see a live image and offers the following additional options:
• Auto-exposure to region – set area that you want the autoexpose to focus on.
• Capture selected region – capture an image of a selected region of interest.
11. Select the Capture button to capture the image according to the set specications.
12. After capture, images will appear in the Gallery automatically. The Gallery can be reached at any
time by pressing the Gallery icon.
13. Go back one screen at any time by pressing the back icon.
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