Azure Imaging System User Manual Page 17
3.6 Protein Gels with Dyes Imaging Mode
Use this for protein gel applications. There are different imaging modalities depending on the dye:
• Fluorescent dyes excited with UV light. Sample placed on the transilluminator (e.g.: light-induced
crosslinked UV dyes).
• Fluorescent dyes excited with EPI light (RGB, NIR700, NIR800). Sample placed on top of the Black
Tray (e.g.: SYPRO Ruby, Coomassie Blue uorescence).
• Colorimetric dyes visualized with visible light. Sample placed on top of the Orange Tray or Trans
White Table (Silver Stain, Coomassie Blue).
1. Place sample in the center of the eld of view (UV Transilluminator or Black Tray, depending on the
detection mode).
2. Close the Azure Imaging System door.
3. Select Protein Gels with Dyes from the home screen.
4. By default, the software will be set to gray as the primary channel. Click the gray Choose a Channel
button to select the desired dye for imaging.
Note: List of dyes available is customizable in the Settings tab.
5. Add additional dyes if desired by clicking on any one of the three additional color channels: red,
green, blue, and selecting the desired dye to assign it to that color channel.
6. Use Auto Image if you want the system to determine the optimal default exposure for each selected
channel using the Wide Dynamic Range exposure mode.
7. Select Manually Image to control all imaging settings.
8. Select the Pixel Binning setting based on the desired sensitivity and imaging resolution. See Section
3.2 for more details.
9. Exposure type can be selected for each channel. Select between the different auto exposure modes
or choose Manual Exposure Type to enter the desired exposure time. See Section 3.2 for more details
on the different Auto Exposure options.
10. In Manually Image menu, tap to enable Live Mode. Click again to close. Live Mode illuminates the
imaging area to allow you to see a live image and offers the following additional options.
• Auto-exposure to region – set area that you want the autoexpose to use to determine
exposure time.
• Capture selected region – capture an image of the selected region of interest.
11. UV Override is useful for pre-warming the UV bulbs or for light-induced cross-linking of UV-
activated for total protein staining. Click the UV Override button to engage.
• Select Light On 5 Minutes for UV dye activation. Check the box for automatic image acquisition if
you would like the imager to acquire an image immediately after the 5 minutes.
12. Select the Capture button to capture the image according to the set specications
13. After capture, images will appear in the Gallery automatically. The Gallery can be reached at any
time by pressing the Gallery icon.
14. Go back one screen at any time by pressing the back icon.
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