User’s Guide106
No signal in red
laser channels
(when red laser is
installed)
High event rate
Low event rate
Possible causes Recommended solutions
Incorrect laser delays due to
a change in the sheath tank
fluid level
Check the fluid level in the sheath
tank and refill if necessary.
Adjust the laser delay settings. See
Manual settings (page 111).
Possible causes Recommended solutions
Air bubbles in the sheath
filter or flow cell
Remove the air bubbles. See Removing
air bubbles (page 37).
Threshold level is too low Increase the threshold level. See the
BD FACSDiva Software Reference
Manual for instructions.
PMT voltage for the
threshold parameter is set
too high
Set the PMT voltage lower for the
threshold parameter. See the
BD FACSDiva Software Reference
Manual for instructions.
Sample is too concentrated Dilute the sample.
Sample flow rate is set to
HIGH
Set the sample flow rate to MED or
LOW.
Possible causes Recommended solutions
Threshold level is too high Lower the threshold level. See the
BD FACSDiva Software Reference
Manual for instructions.
Air bubble or debris in the
flow cell
Prime the fluidics system. See Priming
the fluidics (page 42).
PMT voltage for the
threshold parameter is set
too low
Set the PMT voltage higher for the
threshold parameter. See the
BD FACSDiva Software Reference
Manual for instructions.
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