BD FACSymphony A3 - Cytometer Settings Workflow

User’s Guide64
Cytometer settings workflow
Introduction This topic describes how to optimize cytometer settings. The
optimization is performed using the Cytometer Setup and
Tracking, Application Settings, and Compensation Setup features
of BD
FACSDiva software.
When to optimize
settings
Before you record data for a sample, optimize the cytometer
settings for the sample type and fluorochromes used.
Manual
compensation
Compensation setup automatically calculates compensation
settings. If you choose to perform compensation manually, not all
of the following instructions apply. For detailed instructions, see
the BD
FACSDiva Software Reference Manual.
First-time users If you are performing the procedures in this workflow for the first
time, you should be familiar with BD
FACSDiva software
concepts: workspace components, cytometer and acquisition
controls, and tools for data analysis.
For additional details, see the BD FACSDiva Software Reference
Manual.
Before you begin Start the cytometer and perform the setup and QC procedures. See
Cytometer setup (page 33).