Chapter 3: Cytometer setup 37
5. Add 10 L of sheath fluid, such as BD FACSFlow™ solution, to
the sheath container.
Note: The 10 L will reach the interior line on the sheath tank.
Do not fill the sheath tank further.
6. Replace the sheath container lid.
7. Reconnect the green air line and the alarm line.
8. Make sure the gasket on the inside lip of the sheath lid is
seated correctly and has not slipped out of position.
If the gasket is not seated correctly, the tank will not pressurize
properly.
9. Close the sheath lid and tighten the clamp knob to finger-tight.
Ensure that the blue sheath fluid line is not kinked.
More information • Removing air bubbles (page 37)
• Changing the sheath filter (page 56)
• Cleaning or replacing the sheath gasket (page 62)
Removing air bubbles
Introduction This topic describes how to remove trapped air bubbles in the
sheath filter and the sheath line. Air bubbles can occasionally
dislodge and pass through the flow cell, resulting in inaccurate
data.
Note: Perform this activity every time the sheath tank is refilled.
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