Chapter 2: Introduction 31
Signal detectors Light signals are generated as particles pass through the laser beam
in a fluid stream. When these optical signals reach a detector,
electrical pulses are created that are then processed by the
electronics system.
There are two types of signal detectors in the BD FACSymphony
A3 and
BD FACSymphony A5 flow cytometers:
• PMTs. Used to detect the weaker signals generated by side
scatter (S
SC) and all fluorescence channels. These signals are
amplified by applying a voltage to the PMTs.
• Photodiodes. Less sensitive to light signals than the PMTs. A
p
hotodiode is used to detect the stronger forward scatter (FSC)
signal.
Workstation
Introduction This topic describes the components of the workstations.
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