BD FACSymphony A3 - Optics

Chapter 2: Introduction 29
Optics
Introduction This topic describes the optical components for the
BD
FACSymphony A3 and BD FACSymphony A5 flow cytometers
including:
• Detector arrays
• Laser options
• Optical filters
• Signal detectors
Detector arrays The detector arrays consist of decagons or cascade arrays. Each
decagon can be outfitted with two to 10 photomultiplier tubes
(PMTs) and can detect the corresponding number of signals. Each
cascade array can be outfitted with two to 20 PMTs and can detect
the corresponding number of signals.
Laser options The flow cytometers can be configured with up to five lasers on the
BD
FACSymphony A3 or 10 lasers on the BD FACSymphony A5.
The laser wavelengths and powers for your instruments can be
found on the labels of the laser modules or in the BD
FACSDiva
software under Configurations. The following table lists some of
the common lasers.
Other optional lasers include 375 nm, 420 nm, 445 nm, 458 nm,
460
nm, 473 nm, 505 nm, 514 nm, 532 nm, 552 nm, 568 nm,
588
nm, 592 nm, 628 nm, 640 nm, 647 nm, 660 nm, 685 nm,
730
nm, 785 nm, and 980 nm. Depending on the laser, power
Laser Wavelength (nm) Power (mW)
Violet 405 100
Blue 488 100
UV 355 100
Yellow Green 561 100
Red 637 140