User’s Guide112
About laser delay
Introduction This topic describes how to manually set the laser delay if you are
not using CS&T for cytometer setup and tracking.
About laser signal
delay
Sample interrogation takes place within the cuvette flow cell. Laser
light is directed through a series of prisms that focus multiple lasers
on the event stream at different positions. This allows optimal
detection of fluorescent signals from each laser with minimal cross-
contamination from the other beams.
For example, in a four-laser system, the
blue laser intercepts the
stream first, followed by the violet, UV, and red lasers. Because the
laser signals are spatially separated, there is a slight delay between
the detection of each laser’s signal.
Time
Red
Event intercepts blue laser
Event intercepts violet laser
Event intercepts UV laser
Event intercepts red laser
Event intercepts yellow-green laser*
Yellow-green
*The yellow-green laser is only available through the BD special order research program.
Blue
UV
Violet
The laser delay setting in BD FACSDiva software is used to re-align
the signals so they can be measured and displayed on the same time
scale. Signals are aligned with respect to the blue laser, so the blue
laser will have a 0 delay value, and the red laser will have the
longest delay.
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