Chapter 7: Troubleshooting 109
High CV or poor QC
results
Possible causes Recommended solutions
Air bubble in sheath filter or
flow cell
Purge the filter. See Removing air
bubbles (page 37).
Prime the fluidics system. See
Priming the fluidics (page 42).
Sample flow rate is set too
high
Set the sample flow rate lower.
Air leak at sheath container Ensure that the sheath container lid is
tight and all connectors are secure.
Flow cell is dirty Flush the system. See Flushing the
system (page 52).
Waste tank is pressurized Replace the waste container cap. See
Replacing the waste container cap
(page 54).
Poor sample preparation Repeat sample preparation.
Sample was not diluted in the
same fluid as the sheath fluid
Dilute the sample in the same fluid as
you are using for sheath.
Old or contaminated QC
particles
Make new QC samples and perform
the quality control procedure again.
Instrument is not warmed up Wait 30 minutes for the instrument to
warm up.
Laser is not functioning Contact BD Biosciences.
Optical alignment problem Contact BD Biosciences.
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