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BD FACSymphony A3 - Page 82

BD FACSymphony A3
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User’s Guide82
Compensation control tubes are added to the experiment.
Worksheets containing appropriate plots and gates are added
for each compensation tube.
Recording
compensation
settings
To record compensation settings:
1. Press RUN and HIGH on the cytometer fluid control panel.
2. Install the unstained control tube onto the SIP.
3. Expand the Compensation Controls specimen in
the Browser.
4. Set the current tube pointer to the unstained control tube (it
becomes
green), then click Acquire Data in the Acquisition
Dashboard.
5. Verify that the population of interest is displayed appropriately
on the
FSC vs SSC plot and adjust voltages if necessary.

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