Chapter 7: Troubleshooting 107
Erratic event rate
Sample is not adequately
mixed
Mix the sample to suspend the cells.
Sample is too diluted Concentrate the sample. If the flow
rate setting is not critical to the
application, set the flow rate switch to
MED or HIGH.
Sample injection tube is
clogged
Remove the sample tube to allow
backflushing.
If the event rate is still erratic, clean
the sample injection tube. See Cleaning
the fluidics (page 49).
Possible causes Recommended solutions
Possible causes Recommended solutions
Sample tube is cracked Replace the sample tube.
Air bubble or debris in the
flow cell
Prime the fluidics system. See Priming
the fluidics (page 42).
Bal seal is worn Replace the Bal seal. See Changing the
Bal seal (page 58).
Sample injection tube is
clogged
Remove the sample tube to allow
backflushing.
If the event rate is still erratic, clean
the sample injection tube. See Cleaning
the fluidics (page 49).
Contaminated sample Prepare the specimen again. Ensure
that the tube is clean.
Sheath filter is dirty Replace the filter. See Changing the
sheath filter (page 56).
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