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General| Type | Flow Cytometer |
|---|---|
| Data Acquisition Rate | Up to 40, 000 events per second |
| Software | BD FACSDiva Software |
| Cell Sorting Capability | No |
| Laser Configuration | Up to 5 lasers |
| Detectors | Up to 50 detectors |
| Forward Scatter (FSC) Detection | Yes |
| Side Scatter (SSC) Detection | Yes |
| Sample Volume | 96-well plates |
| Sample Throughput | High throughput capabilities |
| Applications | Immunophenotyping, cell cycle analysis, apoptosis |
Describes the procedures necessary to operate and maintain the BD FACSymphony™ A5 SE flow cytometer.
Lists the safety symbols used in the guide to alert you to potential hazards.
Describes the documentation available with the BD FACSymphony™ A5 SE flow cytometer.
Describes how to get technical assistance and contact BD Biosciences.
Describes the BD FACSymphony™ A5 SE system components, including the flow cytometer, software, and optional accessories.
Shows and describes the main components of the instrument with numbered labels.
Overviews the components of the control panel and lists their functions.
Explains the fluidics system, including system indicators and fluid control buttons.
Describes the optical components, including detector arrays, laser options, and optical filters.
Describes the components of the workstations, including the PC and operating system.
Provides a step-by-step procedure for starting the cytometer and its associated computer.
Describes how to prepare the sheath container for use with the cytometer.
Details how to remove trapped air bubbles from the sheath filter and sheath line to ensure accurate data.
Describes how to prepare the waste container to prevent overflow and ensure safe handling of waste.
Describes how to prepare the fluidics system, including priming procedures to remove air and debris.
Explains the procedure for adding a bead lot number, requiring administrator privileges.
Explains how to create custom configurations and define baselines using BD Cytometer Setup Software (CS&T).
Provides an overview of routine cytometer maintenance and cleaning procedures, including general guidelines.
Describes how to perform daily fluidics cleaning to prevent clogging and remove residual dyes.
Provides instructions on how to properly shut down the cytometer and computer.
Details how to perform an overall fluidics cleaning to remove debris and contaminants from tubing and the flow cell.
Describes the procedure for replacing the waste container cap, recommended monthly.
Explains how to change the sheath filter assembly, recommended every six months.
Describes how to replace the Bal seal, a ring that forms a seal with the sample tube.
Describes how to clean or replace the gasket on the sheath tank lid when needed.
Introduces spectral flow cytometry as an alternative measurement strategy and its capabilities on the BD FACSymphony™ A5 SE.
Lists common dyes and the primary or secondary detector parameters to use with them.
Explains autofluorescence as the intrinsic fluorescent signal produced by cells and its role in spectral unmixing.
Discusses viewing compensated (non-spectral) data and data views in BD FACSDiva™ software for spectral unmixing.
Guides users through creating a new experiment, specimen, and verifying configuration in BD FACSDiva™.
Details the steps for creating a spectral unmixing matrix before performing compensation setup.
Guides users on recording sample data and creating plots, gates, and statistics for analysis.
Outlines the workflow for optimizing cytometer settings using BD software features.
Describes how to verify cytometer configuration and user preferences before creating an experiment for accurate data.
Explains how to run a performance check as part of quality control to monitor instrument performance.
Guides on creating a new experiment, specifying parameters, and adding compensation tubes.
Describes how to create application settings for consistent and reproducible cytometer configurations.
Explains how to record compensation settings using unstained and single-color control tubes.
Describes how to calculate compensation settings and link them to cytometer settings.
Outlines basic acquisition and analysis tasks using BD FACSDiva™ software.
Describes how to prepare the workspace and apply application settings to an experiment before recording data.
Provides an example of how to preview and record data for multiple samples.
Guides on analyzing recorded tubes by creating plots, gates, and statistics views.
Describes how to apply analysis to multiple tubes using global worksheets.
Explains laser signal delay and how it affects signal measurement and alignment.
Guides on optimizing laser delay using BD FACSDiva™ software for proper event assignment.
Describes how to manually adjust area scaling for applications, especially for larger particles.
Addresses common cytometer issues like visible droplets on SIP and sample tube fitting problems.
Lists possible causes and solutions for the issue of no events displayed with the RUN button green.
Addresses issues related to incorrect fluorochrome assignment or non-functioning lasers.
Provides solutions for issues causing a high event rate, such as air bubbles or low thresholds.
Covers issues with distorted scatter parameters due to improper adjustments or dirty components.
Focuses on electronics issues, specifically the 'Cytometer Disconnected' error in the cytometer window.
Provides contact information for ordering spare parts and consumables from BD Biosciences.
Lists available QC and CS&T beads with their supplier and catalog numbers.
Lists essential reagents like sheath fluid, detergent, and dyes with supplier and catalog numbers.
Lists necessary equipment items like Bal seals and sheath filter assemblies with supplier and catalog numbers.
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