No events in acquisition display and RUN button is orange
Possible causes Recommended solutions
RUN is not activated Press the RUN button.
Sample tube is not installed or is not
properly seated
Install the sample tube correctly on the SIP.
Sample tube is cracked Replace the sample tube.
Waste tubing line is disconnected from
the waste container tank
Connect the waste tubing line to the waste container tank.
Sheath container is not pressurized
l
Ensure that the sheath container lid and all connectors are securely seated.
l
Inspect the sheath container O-ring inside the lid and replace it if necessary.
See Cleaning or replacing the sheath gasket (page 45).
Bal seal is worn Replace the Bal seal. See Changing the Bal seal (page 42).
Air leak at sheath container Ensure that the sheath container lid and all connectors are securely seated.
Sheath container is empty Fill the sheath container.
Air in sheath filter Purge the filter. See Removing air bubbles (page 27).
No fluorescence signal
Possible causes Recommended solutions
Incorrect fluorochrome
assignment
Make sure that the cytometer configuration in the software matches the optical filters in the
cytometer and the configuration is as expected.
Laser is not functioning Call your BD service representative.
No signal in red laser channels (when red laser is installed)
Possible causes Recommended solutions
Incorrect laser delays due to a change in the sheath tank fluid
level
l
Check the fluid level in the sheath tank and refill if
necessary.
l
Adjust the laser delay settings. See Manual settings
(page 93).
106 BD FACSymphony™ A5 SE User's Guide
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