Maintenance overview
Introduction
This topic provides an overview of the flow cytometer routine maintenance and cleaning procedures.
General use guidelines
Contact with biological specimens and materials can transmit potentially fatal disease.
Follow these guidelines whenever operating or maintaining the cytometer:
l
Wear suitable protective clothing, eyewear, and gloves.
l
Handle all biological specimens and materials in accordance with applicable regulations and manufacturer
specifications.
l
Dispose of waste using proper precautions and in accordance with local regulations.
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Never pipette by mouth.
For fluidics maintenance, we recommend the following cleaning solutions:
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BDFACSClean™
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BD
®
Detergent Solution Concentrate
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10% bleach solution
Use DI water to dilute bleach to appropriate concentrations.
Higher concentrations of sodium hypochlorite and use of other cleaning solutions might damage the cytometer.
When to perform maintenance procedures
Perform maintenance procedures in the following frequencies.
Frequency Maintenance procedure
Daily
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Cleaning the fluidics (page 37)
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Shutting down the cytometer (page 38)
Scheduled (every 2 weeks)
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Flushing the system (page 38)
Periodic (frequency depends on how often you run the cytometer)
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Changing the sheath filter (page 41)
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Changing the Bal seal (page 42)
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Changing the sample tube O-ring (page 44)
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Cleaning or replacing the sheath gasket (page
45)
36 BD FACSymphony™ A5 SE User's Guide