BD FACSymphony A5 SE - Data recording and analysis workflow

Data recording and analysis workflow
Introduction
This topic outlines the basic acquisition and analysis tasks using BDFACSDiva™ software.
About the examples
The examples in this chapter are from two 4-color bead samples with the following fluorochromes:
l
FITC
l
PE
l
PerCP
l
APC
If you use a different sample type or if you have skipped the optimization steps in Optimizing cytometer
settings (page 67), your software window content, names of folders and experiments, and your data plots and
statistics might differ from those shown here. You might also need to modify some of the instructions in the
procedure.
For additional details on completing some of the following steps, see the BDFACSDiva™ Software Reference
Manual.
This procedure builds on the results obtained in Optimizing cytometer settings (page 67).
Workflow for recording and analyzing data
Recording and analyzing data consists of the following steps.
Step Description
1 Preparing the workspace (page 84)
2 Recording data (page 85)
3 Analyzing data (page 87)
4 Reusing an analysis (page 91)
Preparing the workspace
Introduction
This topic describes how to prepare the workspace and apply application settings to your experiment before
recording data.
84 BD FACSymphony™ A5 SE User's Guide