3. Direct the vent line into a beaker and press the small button at the end of the vent fitting against the side of
the beaker until a steady stream of fluid empties from the filter.
Item Number Component
1 Button
2 Vent fitting
4. Tilt the filter and verify that no trapped air remains in the filter.
5. Repeat steps 3 and 4 until no air is observed in the filter.
6. Check the sheath line for air bubbles.
7. Open the roller clamp at the fluidics interconnect (if necessary) to bleed off any air in the line. Collect any
excess fluid in a waste container.
8. Close the roller clamp.
Preparing the waste container
Introduction
This topic describes how to prepare the waste container. Prevent waste overflow by emptying the waste
container daily or whenever the system status indicator turns yellow.
Note: If your system is connected to the FFSS, see the documentation provided with your FFSS.
All biological specimens and materials coming into contact with them are considered biohazardous. Handle as if
capable of transmitting disease. Dispose of waste using proper precautions and in accordance with local
regulations. Never pipette by mouth. Wear suitable protective clothing, eyewear, and gloves.
Chapter 3 Cytometer setup 29
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