5. If the CSTMismatch dialog opens, click Use CSTSettings.
6. Select Edit > User Preferences.
7. Click the General tab and select the Load data after recording checkbox.
See theBDFACSDiva™ Software Reference Manual for more information about cytometer configurations and
user preferences.
Next step
Running a performance check (page 71)
More information
l
Setting up a compensation experiment (page 74)
Running a performance check
Introduction
This topic describes how to run a performance check as part of quality control.
Overview
The CS&T application is designed to monitor performance on a daily basis and to optimize laser delay.
Running a performance check on a regular basis provides a standard for monitoring changes in performance
due to degradation of laser power, aging of PMTs, and other potential cytometer service issues. Performance
results are also affected by fluidics performance. We strongly recommend following the fluidics maintenance
procedures as described in Cleaning the fluidics (page 37).
Considerations
Some BP filters might not be normalized to CS&T settings. In this case, CS&T will generate Qr and Br numbers
that are not comparable from instrument to instrument. They are however, still trackable on one cytometer.
Part of the process for optimizing cytometer settings includes verifying PMT voltages set by CS&T for all
parameters.
Before you begin
Define the performance baseline for any configuration before running a performance check. See Custom
configurations and baselines (page 34).
Chapter 6 Optimizing cytometer settings 71
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