BD FACSymphony A5 SE - Shutting down the cytometer; Flushing the system

Shutting down the cytometer
Introduction
This topic describes how to shut down the cytometer.
Before you begin
Each time you shut down the cytometer, perform the daily cleaning as described in Cleaning the fluidics (page
37).
Procedure
To shut down the cytometer:
1. Place a tube of DI water on the SIP.
2. Turn off the flow cytometer.
3. Select Start > Shutdown to turn off the computer (if needed).
4. If your system is connected to the FFSS, shut off the FFSS.
If the cytometer will not be used for a week or longer, perform a system flush and leave the fluidics system filled
with DI water to prevent saline crystals from clogging the fluidics.
More information
l
Cleaning the fluidics (page 37)
l
Flushing the system (page 38)
Flushing the system
Introduction
This topic describes how to perform an overall fluidics cleaning to remove debris and contaminants from the
sheath tubing, waste tubing, and flow cell. Perform the system flush at least every 2weeks.
Note: After installation of a new system or major repair of the fluidics system, perform a system flush with
overnight soak twice a week for one month.
Note: If you are using the FFSS, see the BDFACSFlow™ Supply System User’s Guide for instructions on flushing
the system.
The cytometer hardware might be contaminated with biohazardous material. Use 10% bleach to decontaminate
the instrument.
38 BD FACSymphony™ A5 SE User's Guide