BD FACSymphony A5 SE - Sheath and Waste Containers

Droplet containment module
The droplet containment module (DCM) prevents sheath fluid from dripping from the SIP and provides
biohazard protection.
Note: The DCMwill not run when the HTS selector switch is in Plate Mode.
When no sample tube is installed on the SIP, sheath fluid backflushes through the sample injection tube. This
backflush helps prevent carryover of cells between samples. The DCM vacuum is activated when the sample
tube is removed and the tube support arm is moved to the side. Sheath fluid is aspirated as it backflushes the
sample injection tube.
Sheath and waste containers
Introduction
This topic describes the sheath and waste containers. The sheath and waste containers are outside the
cytometer and are positioned on the floor. The sheath and waste containers should remain in the installed
position. Moving them to a different height (for example, from the floor to a bench) changes the flow rate.
Note: If you are using the FFSS, see the BDFACSFlow™ Supply System User’s Guide.
Sheath container
The sheath container has a capacity of 10L. Sheath fluid is filtered through an in-line, interchangeable filter
that prevents small particles from entering the sheath fluid lines. An alarm sounds when the container is empty.
Do not fill the sheath tank to its maximum capacity. When an overfull tank is pressurized, erratic cytometer
performance can result.
Waste container
The waste container has a capacity of 10L. An alarm sounds when the container is full. If the waste container
overfills, install a replacement waste tank cap and baffle.
More information
l
Preparing the sheath container (page 25)
l
Preparing the waste container (page 29)
l
Status screen (page 16)
l
Fluidic alarms and the Mode button (page 17)
Chapter 2 Introduction 19