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BD FACSymphony A5 SE - Page 17

BD FACSymphony A5 SE
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Item Definition
1 Waste level. Shows range (1) from E (empty) to F (full). The display line increases from left to right in sequences
of 20%. System status turns yellow at 80%, and red at 100% full.
2 Sheath level. Shows range from E to F. The display line decreases from right to left in sequences of 20% from
full level. System status turns yellow at 20%, and red at 0%.
3 Fine Adj. Shows the current setting of fine adjust. Fine adjust can be set in increments of 10 from 0 to 500. The
last value persists, even after cytometer shutdown.
Air pressure. Shows the air pressure as either greater than (>) 5.4 psi, or less than (<) 5 psi. To display psi, press
the Mode button, followed by the Up button.
4 FFSS Mode. Shows that the cytometer is in FFSS mode. To enter this mode, press the MODE button 4times.
After you reach FFSS OFF, push the SAMPLE ADJ button once. You should now see the panel display FFSS ON. To
exit, press the MODE button 4 times, then SAMPLE ADJ button once. Repeat the process to turn FFSS mode off.
Fluidic alarms and the Mode button
The fluidic alarms are triggered by the waste and sheath fluid levels in the tanks. The alarms sound when the
waste tank is nearly 100% full and the sheath tank is empty.
To silence the alarm, press the Mode button, then press the Down button. The Mode button flashes to indicate
the cytometer is in silent mode. Repeat this sequence to turn off silent mode.
Note: When the cytometer is in FFSS mode, the alarms are deactivated.
Sample injection port
The SIP is where the sample tube is installed. The SIP includes the sample injection tube and the tube support
arm. Samples are introduced through a stainless steel injection tube equipped with an outer droplet
containment sleeve. The sleeve works in conjunction with a vacuum pump to eliminate droplet formation of
sheath fluid as it backflushes from the sample injection tube.
Chapter 2 Introduction 17

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