Optimizing laser delay
Introduction
This topic describes how to optimize the laser delay using BDFACSDiva™ software.
Before you begin
To optimize the delay for a given laser, you must acquire events from a sample with a fluorescence signal
excited by that laser. Follow the procedures in Recording and analyzing data (page 83), for sample optimization
and acquiring data.
Procedure
To optimize laser delay:
1. While acquiring data from your sample, create a histogram to show the fluorescence signal excited by the
laser for which the delay is to be optimized.
2. In the Acquisition Dashboard, set the Events to Display to 500 events.
3. Click the Laser tab in the Cytometer window.
Window extension and laser delay values are displayed in microseconds (µs).
4. Set the Window Extension value to 0 µs.
Chapter 8 Manual settings 95
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