Calculating compensation
Introduction
This topic describes how to calculate compensation.
Before you begin
Before you can calculate compensation, you need to record the data for each single-stained control.
Procedure
To calculate compensation:
1. Select Experiment > Compensation Setup > Calculate Compensation.
Note: If the calculation is successful, a dialog prompts you to enter a name for the compensation setup. The
default name is year/month/day/time.
2. Enter a setup name and click Link & Save to link the compensation to the cytometer settings and save the
settings to the catalog.
To help track compensation setups, include the experiment name, date, or both in the setup name.
The compensation setup is linked to the MyExperiment cytometer settings, and subsequent acquisitions in
MyExperiment are performed with the new compensation settings.
We recommend that you always visually and statistically inspect automatically calculated spectral overlap
values. The means of the positive controls should be aligned with the means of the negative controls.
More information
l
Recording compensation controls (page 78)
Chapter 6 Optimizing cytometer settings 81
To Next Page
Loading...
