BD FACSymphony A5 SE - Recording and analyzing spectral data

Note: To view the saved settings, select menu item Cytometer >Catalogs and select the
CompensationSetup tab. The Type column on this tab classifies settings created using Calculate Spectral
Unmixing as Spectral and settings created using Calculate Compensation as Compensated.
You can elect to switch between spectral unmixed and traditional compensation values, by toggling between
Spectral and Compensated in the dropdown menu, to compare the plot data in each case.
Recording and analyzing spectral data
1. If needed, with the specimen tube selected, create more plots, gates, and statistics in addition to those
created when initially setting up the experiment.
2. Record sample data using the initial tube.
3. To acquire and record more tubes, click Next Tube and/or Duplicate the Specimen. Tubes must have the
same parameter labels as was set up in the Spectral Unmixing algorithm by the Create Compensation
Controls menu command.
62 BD FACSymphony™ A5 SE User's Guide