BD FACSymphony A5 SE - Cytometer troubleshooting

Cytometer troubleshooting
Introduction
This topic describes possible problems and recommended solutions for BDFACSymphony™A5SE flow
cytometer issues.
Droplets are visible on the SIP
Possible causes Recommended solutions
Worn O-ring in the retainer Replace the O-ring. See Changing the sample tube O-ring (page 44).
Outer sleeve is not seated in the retainer 1. Loosen the retainer.
2. Push the outer sleeve up into the retainer until seated.
3. Tighten the retainer.
Outer sleeve is not on the sample injection
tube
Replace the outer sleeve.
1. Loosen the retainer.
2. Slide the outer sleeve over the sample injection tube until it is seated.
3. Tighten the retainer.
Waste line is pinched, preventing proper
aspiration
Check the waste line.
Waste tank is full Empty the waste tank.
Droplet containment vacuum is not
functioning
Call your BD service representative.
The mode is not set to HTS. Change the mode to HTS by pressing down the Mode button for more than
3seconds.
Sample tube not fitting on SIP
Possible causes Recommended solutions
Sample tube other than Falcon
®
tubes used Use Falcon
®
12 x 75-mm sample tubes. See Equipment (page 113).
Worn Bal seal Replace the Bal seal. See Changing the Bal seal (page 42).
Sample tube is cracked Transfer contents to a new tube.
Rapid sample aspiration
Possible causes Recommended solutions
Support arm is to the side Place the support arm under the sample tube.
Droplet containment
module is failing
Try the solutions in Droplets are visible on the SIP (page 104). If the issue is not resolved,
call your BD service representative.
104 BD FACSymphony™ A5 SE User's Guide