Adjusting area scaling
Introduction
This topic describes how to manually adjust the area scaling on your cytometer if necessary for your
application. The area scaling is automatically set in CS&T. Depending on the size of your target particle, you
might need to adjust the area scaling manually. Larger particles are more likely to require an area scaling
adjustment.
About area scaling
The area of a pulse is calculated by BDFACSDiva™ using measured height and width measurements. It is
sometimes important to verify that the area calculation and the height measurement are equivalent by
adjusting the factor applied to the area. The required area scaling factor changes based on sheath pressure
and particle size.
About this example
The following example describes how to adjust area scaling for an experiment which uses only the 488-nm laser
and the 640-nm laser. You must adjust area scaling for all lasers used in your experiment. To adjust the other
lasers, add a parameter and the corresponding plots from that laser to the procedure.
Procedure
To adjust area scaling:
1. Open an existing experiment, or create a new experiment in the Browser.
2. Create a new specimen by clicking the New Specimen button on the Browser toolbar.
Chapter 8 Manual settings 97
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