11. Before recording, preview the data on the MyData worksheet to verify that all expected populations are
visible and the data is similar to the previous sample.
12. Click Record Data.
13. When event recording has completed, remove the second tube from the cytometer.
14. If you are recording more than two tubes, repeat steps 8 through 13 for the remaining tubes.
15. Print the experiment-level cytometer settings by right-clicking the Cytometer Settings icon in the Browser
and selecting Print.
16. Install a tube of DI water onto the SIP.
17. Place the cytometer in standby mode.
More information
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Analyzing data (page 87)
Analyzing data
Introduction
This topic describes how to analyze recorded tubes by creating plots, gates, a population hierarchy, and
statistics views on a new global worksheet.
Analyzing data
To analyze data:
1. Use the Browser toolbar to create a new global worksheet. Rename it MyDataAnalysis.
2. Create the following plots on the MyDataAnalysis worksheet:
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FSC vs SSC
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FITC vs PE
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FITC vs PerCP
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FITC vs APC
3. Create a population hierarchy and a statistics view, and set them below the plots on the worksheet.
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Right-click any plot and select Show Population Hierarchy.
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Right-click any plot and select Create Statistics View.
4. Set the current tube pointer to Beads_001.
5. Draw a gate around the singlets on the FSC vs SSC plot.
6. Use the population hierarchy to rename the population Singlets.
Chapter 7 Recording and analyzing data 87
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