BD FACSymphony A5 SE - Recording data

Note: If you need to run samples at an event rate greater than 10,000 events/second, consider changing your
Window extension. See the BDFACSDiva™ Software Reference Manual for more information.
Recording data
To record data:
1. Press RUN and HIGH on the cytometer fluid control panel.
2. Install the first sample tube onto the SIP.
3. Set the current tube pointer to Beads_001.
4. Click Acquire Data in the Acquisition Dashboard to begin acquisition.
5. While data is being acquired:
a. Draw a gate around the singlets on the FSC vs SSC plot.
b. Rename the P1 gate to Singlets.
c. Use the Inspector to set the other plots to show only the singlet population by selecting the Singlets
checkbox.
6. Click Record Data.
7. When event recording has completed, remove the first tube from the cytometer.
The MyData worksheet plots should look like the following.
8. Install the second sample tube onto the SIP.
9. Set the current tube pointer to Beads_002.
10. Click Acquire Data to begin acquisition.
86 BD FACSymphony™ A5 SE User's Guide