BD FACSymphony A5 SE - Electronics troubleshooting

High CV or poor QC results
Possible causes Recommended solutions
Air bubble in sheath filter or flow cell
l
Purge the filter. See Removing air bubbles (page 27).
l
Prime the fluidics system. See Preparing the fluidics (page 31).
Sample flow rate is set too high Set the sample flow rate lower.
Air leak at sheath container Ensure that the sheath container lid is tight and all connectors are
secure.
Flow cell is dirty Flush the system. See Flushing the system (page 38).
Waste tank is pressurized Replace the waste container cap. See Replacing the waste container
cap (page 40).
Poor sample preparation Repeat sample preparation.
Sample was not diluted in the same fluid as the
sheath fluid
Dilute the sample in the same fluid as you are using for sheath.
Old or contaminated QC particles Make new QC samples and perform the quality control procedure
again.
Instrument is not warmed up Wait 30 minutes for the instrument to warm up.
Laser is not functioning Contact BDBiosciences.
Optical alignment problem Contact BDBiosciences.
Carryover from previous sample observed
Possible causes Recommended solution
Residual sample in sample injection tube or
fluidic system from previous tests
Clean the sample injection tube and, if carryover is still observed, clean
the fluidics (see Cleaning the fluidics (page 37).
Electronics troubleshooting
Introduction
This topic describes possible problems and recommended solutions for electronics issues.
“Cytometer Disconnected” in cytometer window
Possible causes Recommended solutions
Cytometer power is off Turn on the cytometer main power.
Communication failure between
workstation and cytometer
1. In BDFACSDiva™ software, select Cytometer > Connect.
2. If connecting does not work, restart the cytometer. Turn the cytometer off,
wait 1 minute, and turn on the cytometer main power.
3. If connecting still does not work, contact BDBiosciences.
Chapter 9 Troubleshooting 109