Distorted scatter parameters
Possible causes Recommended solutions
Cytometer settings are improperly
adjusted
Optimize the scatter parameters. See the BDFACSDiva™ Software Reference
Manual for instructions.
Air bubble in the sheath filter or
flow cell
Purge the air from the filter. See Removing air bubbles (page 27).
Flow cell is dirty Flush the system. See Flushing the system (page 38).
Air leak at sheath container Ensure that the sheath container lid is tight and all connectors are secure.
Hypertonic buffers or fixative Replace the buffers or fixative.
Excessive amount of debris in display
Possible causes Recommended solutions
Threshold level is too low Increase the threshold level.
Sheath filter is dirty Replace the filter. See Changing the sheath filter (page 41).
Flow cell is dirty Flush the system. See Flushing the system (page 38).
Dead cells or debris in the
sample
Examine the sample under a microscope.
Sample is contaminated Re-stain the sample. Ensure that the tube is clean.
Stock sheath fluid is
contaminated
Rinse the sheath container with DI water, then fill the container with sheath fluid from
another (or new lot) bulk container.
108 BD FACSymphony™ A5 SE User's Guide
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